
{"id":42473,"date":"2025-02-05T10:41:49","date_gmt":"2025-02-05T09:41:49","guid":{"rendered":"https:\/\/www.btitrainingcenter.com\/effect-of-implant-abutment-surface-treatments-on-bacterial-biofilm-composition-and-structure\/"},"modified":"2025-07-31T13:41:00","modified_gmt":"2025-07-31T12:41:00","slug":"effect-of-implant-abutment-surface-treatments-on-bacterial-biofilm-composition-and-structure","status":"publish","type":"post","link":"https:\/\/www.btitrainingcenter.com\/pt-pt\/effect-of-implant-abutment-surface-treatments-on-bacterial-biofilm-composition-and-structure\/","title":{"rendered":"Effect of implant abutment surface treatments on bacterial biofilm composition and structure"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row content_text_aligment=&#8221;left&#8221; row_background_lines=&#8221;no&#8221;][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3 class=\"title\">Abstract<\/h3>\n<div id=\"enc-abstract\" class=\"abstract-content selected\">\n<div id=\"enc-abstract\" class=\"abstract-content selected\">\n<p><strong class=\"sub-title\">Background:\u00a0<\/strong>For the long-term success of dental implants, implant abutment surface should promote the attachment of oral epithelial cells and reduce bacterial adhesion. Titanium nitride (TiN) coatings show antimicrobial properties. Nevertheless, there is a lack of clinical trials that assess the biofilm formation on TiN abutments in the context of clinical practice. Thus, the objective of this study was to evaluate the effect of different abutment surfaces (machined, TiN and TiN oxidized) on bacterial biofilm composition and structure.<\/p>\n<p><strong class=\"sub-title\">Materials and methods:\u00a0<\/strong>Implant abutments were connected to the dental implants. Bacterial communities were sampled at 1 and 60 days later. The relationship between surface, periodontal indices and bacterial community dynamics was assessed using 16S rRNA metagenomics. A total of 17 patients were involved in this study (14 included in final analyses: 15 machined, 16 TiN and 14 TiN oxidized abutments).<\/p>\n<p><strong class=\"sub-title\">Results:\u00a0<\/strong>No significant differences between surfaces were found considering taxa abundance, most alpha diversity metrics or community structure. Time showed a significant effect on diversity and also on the abundance of several bacterial taxa.<\/p>\n<p><strong class=\"sub-title\">Conclusions:\u00a0<\/strong>These results indicate that the effect of the three tested abutment surfaces on biofilm structure and composition was negligible, whereas patient and time exert strong influences on bacterial biofilm formation at different scales.<\/p>\n<\/div>\n<p><strong class=\"sub-title\">Keywords:\u00a0<\/strong>Biofilm; bacterial community; dental implant abutment; diversity; metagenomics; surface modifications.<\/p>\n<\/div>\n<p>[\/vc_column_text][vc_empty_space][vc_column_text]<\/p>\n<h3>Figures<\/h3>\n<p>[\/vc_column_text][vc_empty_space][\/vc_column][\/vc_row][vc_row content_placement=&#8221;middle&#8221; content_text_aligment=&#8221;center&#8221;][vc_column]<div class=\"qodef-image-gallery qodef-grid-list qodef-disable-bottom-space  qodef-ig-grid-type qodef-four-columns qodef-tiny-space  qodef-image-behavior-lightbox\">\n\t<div class=\"qodef-ig-inner qodef-outer-space\">\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0001_OC.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-233]\" title=\"&lt;strong&gt;Figure 1.&lt;\/strong&gt; Macrophotographs (1) and scanning electron microscopy (SEM) microphotographs (2) representing the transepithelial abutment surfaces included in the present study: A) as machined (surface A), B) TiN (surface B) and C) Anodized TiN (surface C).\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0001_OC-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0002_OC.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-233]\" title=\"&lt;strong&gt;Figure 2.&lt;\/strong&gt; Flowchart of the randomized clinical trial.\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0002_OC-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0003_B.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-233]\" title=\"&lt;strong&gt;Figure 3.&lt;\/strong&gt; Boxplots of periodontal indices across time and p values obtained after computing repeated measures ANOVA and Bonferroni adjustment for multiple comparisons: A) OLI, B) GI, C) BoP and D) SLI.\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0003_B-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0004_OC.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-233]\" title=\"&lt;strong&gt;Figure 4.&lt;\/strong&gt; Mean bacterial species richness in the implant abutments collected 60\u2009days after implant placement.\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0004_OC-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0005_OC.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-233]\" title=\"&lt;strong&gt;Figure 5.&lt;\/strong&gt; Absolute abundances of the top 6 most abundant bacterial species: Porphyromonas pasteri, Prevotella oris, Streptococcus tigurinus, Peptostreptococcus stomatis, Veillonella dispar and Fusobacterium nucleatum in the implant abutments collected 60\u2009days after implant placement.\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0005_OC-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0006_OC.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-233]\" title=\"&lt;strong&gt;Figure 6.&lt;\/strong&gt; Stacked bar graphs representing cumulative abundances of taxa that represent up to 10 most abundant taxa nested by Phylum (only top 10 phyla were plotted) and grouped by sampling method (TSS normalized data) on the abutments collected: 1 (a) and 60\u2009days (b) after abutment placement. The represented taxonomic ranks (Class, Order, Family and Genus) were nested by Phylum.\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0006_OC-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0007_OC.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-233]\" title=\"&lt;strong&gt;Figure 7.&lt;\/strong&gt; Alpha diversity metrics of biofilm samples: A) boxplots of alpha diversity indices comparing the abutments with different surfaces at 1 and 60\u2009days after placement and B) forest plots of standardized beta values from linear mixed effects models constructed to estimate the effect of surface, time and plaque or inflammation related indices on alpha diversity metrics. Alpha diversity metrics were abbreviated as follows: Observed Richness (Observed), Chao1 Index (\u015cChao1), Shannon Diversity Index (H\u2019), Simpson\u2019s Index (D) and Inverse Simpson\u2019s Index (1\/D).\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0007_OC-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0008_OC.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-233]\" title=\"&lt;strong&gt;Figure 8.&lt;\/strong&gt; Dissimilarity network based on Aitchison distance matrix representing the beta-diversity at the OTU level (zeros were replaced via multiplicative simple replacement and k-nearest neighbor was used as sparsification method).\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0008_OC-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0009_OC.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-233]\" title=\"&lt;strong&gt;Figure 9.&lt;\/strong&gt; Bacterial association networks constructed using SparCC correlation coefficients for compositional data retaining OTUs with at least 0.1% of sequencing reads from all the studied surfaces at days 1 (A) and 60 (B). For network sparsification, only edges corresponding to an absolute association greater or equal than 0.7 were represented in order to improve network readability. Differential association networks based on association networks are also provided for all pairwise comparisons.\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/ZJOM_A_2459922_F0009_OC-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n<\/div>[vc_empty_space height=&#8221;50px&#8221;][\/vc_column][\/vc_row][vc_row][vc_column]<a itemprop=\"url\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/11-ZJOM_17_2459922.pdf\" target=\"_blank\"  class=\"qodef-btn qodef-btn-medium qodef-btn-solid btnWebRosa2\"  >\n    <span class=\"qodef-btn-text\">Descarregar artigo<\/span>\n    <\/a>[\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p><strong>J Oral Microbiol. 2025 Feb 5;17(1):2459922.<\/strong><br \/>\nFor the long-term success of dental implants, implant abutment surface should promote the attachment of oral epithelial cells and reduce bacterial adhesion. Titanium nitride (TiN) coatings show antimicrobial properties. Nevertheless, there is a lack of clinical trials that assess the biofilm formation on TiN abutments in the context of clinical practice. Thus, the objective of this study was to evaluate the effect of different abutment surfaces (machined, TiN and TiN oxidized) on bacterial biofilm composition and structure.<\/p>\n","protected":false},"author":2002,"featured_media":42285,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[154],"tags":[],"class_list":["post-42473","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-implantologia-oral-pt-pt"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/posts\/42473","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/users\/2002"}],"replies":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/comments?post=42473"}],"version-history":[{"count":1,"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/posts\/42473\/revisions"}],"predecessor-version":[{"id":42474,"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/posts\/42473\/revisions\/42474"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/media\/42285"}],"wp:attachment":[{"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/media?parent=42473"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/categories?post=42473"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/pt-pt\/wp-json\/wp\/v2\/tags?post=42473"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}