{"id":53986,"date":"2017-04-01T17:56:09","date_gmt":"2017-04-01T16:56:09","guid":{"rendered":"https:\/\/www.btitrainingcenter.com\/autologous-method-for-ex-vivo-expansion-of-human-limbal-epithelial-progenitor-cells-based-on-plasma-rich-in-growth-factors-technology\/"},"modified":"2026-09-16T09:43:55","modified_gmt":"2026-09-16T08:43:55","slug":"autologous-method-for-ex-vivo-expansion-of-human-limbal-epithelial-progenitor-cells-based-on-plasma-rich-in-growth-factors-technology","status":"publish","type":"post","link":"https:\/\/www.btitrainingcenter.com\/it\/autologous-method-for-ex-vivo-expansion-of-human-limbal-epithelial-progenitor-cells-based-on-plasma-rich-in-growth-factors-technology\/","title":{"rendered":"Autologous method for ex vivo expansion of human limbal epithelial progenitor cells based on plasma rich in growth factors technology"},"content":{"rendered":"

[vc_row content_text_aligment=”left” row_background_lines=”no”][vc_column][vc_column_text]<\/p>\n

Abstract<\/h3>\n
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Purpose:\u00a0<\/strong>Develop an autologous culture method for ex vivo expansion of human limbal epithelial progenitor cells (LEPCs) using Plasma Rich in Growth Factors (PRGF) as a growth supplement and as a scaffold for the culture of LEPCs.<\/p>\n

Methods:\u00a0<\/strong>LEPCs were cultivated in different media supplemented with 10% fetal bovine serum (FBS) or 10% PRGF. The outgrowths, total number of cells, colony forming efficiency (CFE), morphology and immunocytochemistry against p63- \u03b1 and cytokeratins 3 and 12 (CK3-CK12) were analyzed. PRGF was also used to elaborate a fibrin membrane. The effects of the scaffold on the preservation of stemness and the phenotypic characterization of LEPCs were investigated through analysis of CK3-CK12, ABCG-2 and p63.<\/p>\n

Results:\u00a0<\/strong> LEPCs cultivated with PRGF showed a significantly higher growth area than FBS cultures. Moreover, the number of cells were also higher in PRGF than FBS, while displaying a better morphology overall. CFE was found to be also higher in PRGF groups compared to FBS, and the p63-\u03b1 expression also differed between groups. LEPCs cultivated on PRGF membranes appeared as a confluent monolayer of cells and still retained p63 and ABCG-2 expression, being negative for CK3-CK12.<\/p>\n

Conclusions:\u00a0<\/strong>PRGF can be used in corneal tissue engineering, supplementing the culture media, even in a basal media without any other additives, as well as providing a scaffold for the culture.<\/p>\n<\/div>\n

Keywords:\u00a0<\/strong>Cell culture techniques; Cell proliferation; Cell transplantation; Corneal epithelium; Culture media; Limbal deficiency; Limbus corneae; PRGF; Plasma rich in growth factors.<\/p>\n<\/div>\n

[\/vc_column_text][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column]\n Accedi all'articolo completo<\/span>\n <\/a>[\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

Ocul Surf. 2017 Apr;15(2):248-256.<\/strong>
\nDevelop an autologous culture method for ex vivo expansion of human limbal epithelial progenitor cells (LEPCs) using Plasma Rich in Growth Factors (PRGF) as a growth supplement and as a scaffold for the culture of LEPCs.<\/p>\n","protected":false},"author":2002,"featured_media":53973,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[157],"tags":[],"class_list":["post-53986","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicina-rigenerativa"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/posts\/53986","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/users\/2002"}],"replies":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/comments?post=53986"}],"version-history":[{"count":1,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/posts\/53986\/revisions"}],"predecessor-version":[{"id":53987,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/posts\/53986\/revisions\/53987"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/media\/53973"}],"wp:attachment":[{"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/media?parent=53986"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/categories?post=53986"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/tags?post=53986"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}