{"id":47870,"date":"2025-04-24T08:44:48","date_gmt":"2025-04-24T07:44:48","guid":{"rendered":"https:\/\/www.btitrainingcenter.com\/platelet-rich-plasma-activation-are-there-differential-effects-in-reproductive-medicine\/"},"modified":"2026-04-10T12:40:15","modified_gmt":"2026-04-10T11:40:15","slug":"platelet-rich-plasma-activation-are-there-differential-effects-in-reproductive-medicine","status":"publish","type":"post","link":"https:\/\/www.btitrainingcenter.com\/it\/platelet-rich-plasma-activation-are-there-differential-effects-in-reproductive-medicine\/","title":{"rendered":"Platelet-rich plasma activation: are there differential effects in reproductive medicine?"},"content":{"rendered":"

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Abstract<\/h3>\n
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The aim of this in vitro short report was to determine whether activation of platelet-rich plasma (PRP) results in differential effects on growth factor release and cell proliferation in reproductive medicine. This study involved PRP from five donors, activated with either CaCl2 (exogenous activation) or type I collagen (mimicking endogenous activation). The release of growth factors (EGF and PDGF-AB) and the proliferative response of human ovarian fibroblasts were analysed. PRP activated with CaCl2 formed stable clots and released statistically significant higher levels of EGF (871 \u00b1 426 pg\/mL) and PDGF-AB (26535 \u00b1 6477 pg\/mL) compared to collagen-activated PRP (EGF: 141 \u00b1 66 pg\/mL, PDGF-AB: 13060 \u00b1 2301 pg\/mL). Additionally, CaCl2-activated PRP induced greater proliferation in ovarian fibroblasts (34.90 \u00b1 17.82 ng\/mL DNA) than collagen-activated PRP (30.75 \u00b1 18.21 ng\/mL DNA). In conclusion, the activation of PRP with CaCl2 results in higher growth factor release and a stronger biological response compared to type I collagen-activation. These findings underscore the importance of standardized PRP activation protocols to enhance clinical outcomes in reproductive medicine.<\/p>\n<\/div>\n

Keywords:\u00a0<\/strong>Assisted reproductive techniques; Growth factors; PRGF; PRP; Platelet-rich plasma; Regenerative medicine.<\/p>\n<\/div>\n

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Figures<\/h3>\n

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J Ovarian Res. 2025 Apr 24;18(1):84.<\/strong>
\nThe aim of this in vitro short report was to determine whether activation of platelet-rich plasma (PRP) results in differential effects on growth factor release and cell proliferation in reproductive medicine. This study involved PRP from five donors, activated with either CaCl2 (exogenous activation) or type I collagen (mimicking endogenous activation). The release of growth factors (EGF and PDGF-AB) and the proliferative response of human ovarian fibroblasts were analysed. PRP activated with CaCl2 formed stable clots and released statistically significant higher levels of EGF (871 \u00b1 426 pg\/mL) and PDGF-AB (26535 \u00b1 6477 pg\/mL) compared to collagen-activated PRP (EGF: 141 \u00b1 66 pg\/mL, PDGF-AB: 13060 \u00b1 2301 pg\/mL). Additionally, CaCl2-activated PRP induced greater proliferation in ovarian fibroblasts (34.90 \u00b1 17.82 ng\/mL DNA) than collagen-activated PRP (30.75 \u00b1 18.21 ng\/mL DNA). <\/p>\n","protected":false},"author":2002,"featured_media":45541,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[157],"tags":[],"class_list":["post-47870","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicina-rigenerativa"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/posts\/47870","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/users\/2002"}],"replies":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/comments?post=47870"}],"version-history":[{"count":3,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/posts\/47870\/revisions"}],"predecessor-version":[{"id":47873,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/posts\/47870\/revisions\/47873"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/media\/45541"}],"wp:attachment":[{"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/media?parent=47870"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/categories?post=47870"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/it\/wp-json\/wp\/v2\/tags?post=47870"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}