
{"id":43619,"date":"2019-02-10T12:20:40","date_gmt":"2019-02-10T11:20:40","guid":{"rendered":"https:\/\/www.btitrainingcenter.com\/relevance-of-topographic-parameters-on-the-adhesion-and-proliferation-of-human-gingival-fibroblasts-and-oral-bacterial-strains\/"},"modified":"2025-10-09T10:48:09","modified_gmt":"2025-10-09T09:48:09","slug":"relevance-of-topographic-parameters-on-the-adhesion-and-proliferation-of-human-gingival-fibroblasts-and-oral-bacterial-strains","status":"publish","type":"post","link":"https:\/\/www.btitrainingcenter.com\/fr\/relevance-of-topographic-parameters-on-the-adhesion-and-proliferation-of-human-gingival-fibroblasts-and-oral-bacterial-strains\/","title":{"rendered":"Relevance of Topographic Parameters on the Adhesion and Proliferation of Human Gingival Fibroblasts and Oral Bacterial Strains"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row content_text_aligment=&#8221;left&#8221; row_background_lines=&#8221;no&#8221;][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3 class=\"title\">Abstract<\/h3>\n<div id=\"enc-abstract\" class=\"abstract-content selected\">\n<div id=\"enc-abstract\" class=\"abstract-content selected\">\n<p>Dental implantology allows replacement of failing teeth providing the patient with a general improvement of health. Unfortunately not all reconstructions succeed, as a consequence of the development of infections of bacterial origin on the implant surface. Surface topography is known to modulate a differential response to bacterial and mammalian cells but topographical measurements are often limited to vertical parameters. In this work we have extended the topographical measurements also to lateral and hybrid parameters of the five most representative implant and prosthetic component surfaces and correlated the results with bacterial and mammalian cell adhesion and proliferation outcomes. Primary human oral gingival fibroblast (gum cells) and the bacterial strains:\u00a0<i>Streptococcus mutans<\/i>,\u00a0<i>Streptococcus sanguinis<\/i>\u00a0and\u00a0<i>Aggregatibacter actinomycetemcomitans<\/i>, implicated in infectious processes in the oral\/implant environment were employed in the presence or absence of human saliva. The results confirm that even though not all the measured surface is available for bacteria to adhere, the overall race for the surface between cells and bacteria is more favourable to the smoother surfaces (nitrided, as machined or lightly acid etched) than to the rougher ones (strong acid etched or sandblasted\/acid etched).<\/p>\n<\/div>\n<\/div>\n<p>[\/vc_column_text][vc_empty_space][vc_column_text]<\/p>\n<h3>Figures<\/h3>\n<p>[\/vc_column_text][vc_empty_space][\/vc_column][\/vc_row][vc_row content_placement=&#8221;middle&#8221; content_text_aligment=&#8221;center&#8221;][vc_column]<div class=\"qodef-image-gallery qodef-grid-list qodef-disable-bottom-space  qodef-ig-grid-type qodef-four-columns qodef-tiny-space  qodef-image-behavior-lightbox\">\n\t<div class=\"qodef-ig-inner qodef-outer-space\">\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.001.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-879]\" title=\"&lt;strong&gt;Figure 1&lt;\/strong&gt; Primary fibroblast-like cells cultured on the test surfaces. Adhesion was measured at 30, 60, and 90 minutes of contact of the cells with the surfaces. Proliferation was measured at 72 hours of culture. Statistical analysis has been performed for each time of cell adhesion and proliferation independently. Different letters between data show statistical differences (ANOVA p&lt;0.05).\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.001-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.002.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-879]\" title=\"&lt;strong&gt;Figure 2&lt;\/strong&gt; Synthesis of the fibroblast markers procollagen I (PC) and fibronectin (FN) on the test surfaces. Synthesis was measured at 7 days of culture. Statistical analysis has been performed for PC and FN synthesis independently. Different letters between data show statistical differences (ANOVA p&lt;0.05).\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.002-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.003.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-879]\" title=\"&lt;strong&gt;Figure 3&lt;\/strong&gt; Bacterial adhesion per unit of area of S. mutans, S. sanguinis, and A. actinomycetemcomitans measured at 60 min of contact on the test surfaces. One set of samples was in contact with artificial saliva only and the other, with natural saliva. Different letters between data show statistical differences (ANOVA p&lt;0.05).\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.003-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.004.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-879]\" title=\"&lt;strong&gt;Figure 4&lt;\/strong&gt; Viability biofilm formation of S. mutans, S. sanguinis and A. actinomycetemcomitans ATCC on nonconditioned samples and conditioned with human natural saliva. Results of growth after 24 hours, measured in relative light units (RLU). Different letters between data show statistical differences (ANOVA p&lt;0.05).\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.004-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.005.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-879]\" title=\"&lt;strong&gt;Figure 5&lt;\/strong&gt; Areal density of bacteria (nb,N) of S. mutans, S. sanguinis, and A. actinomycetemcomitans on the test surfaces. One set of samples was in contact with artificial saliva only and the other, with natural saliva. Different letters between data show statistical differences (ANOVA p&lt;0.05).\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.005-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.006.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-879]\" title=\"&lt;strong&gt;Figure 6&lt;\/strong&gt; Ratio of fibroblasts proliferation, after 60 minutes of contact, to the number of bacteria of S. mutans, S. sanguinis, and A. actinomycetemcomitans in the projected area on the test surfaces. One set of samples was in contact with artificial saliva only and the other, with natural saliva. Different letters between data show statistical differences (ANOVA p&lt;0.05).\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.006-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<div class=\"qodef-ig-image qodef-item-space\">\n\t\t\t\t<div class=\"qodef-ig-image-inner\">\n\t\t\t\t\t\t\t\t\t\t\t<a itemprop=\"image\" class=\"qodef-ig-lightbox\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.007.jpg\" data-rel=\"prettyPhoto[image_gallery_pretty_photo-879]\" title=\"&lt;strong&gt;Figure 7&lt;\/strong&gt; SEM micrographs of the biofilm formation of S. mutans, S. sanguinis, and A. actinomycetemcomitans on Mach and SB+AE surfaces. All images shown were taken at magnification 15000x and were chosen as the best representatives of the amount of biofilm on surfaces.\">\n\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/BMRI2019-8456342.007-200x200.jpg\" alt=\"\" width=\"200\" height=\"200\" \/>\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n<\/div>[vc_empty_space height=&#8221;50px&#8221;][\/vc_column][\/vc_row][vc_row][vc_column]<a itemprop=\"url\" href=\"https:\/\/www.btitrainingcenter.com\/wp-content\/uploads\/2021\/08\/1_-_BMRI2019-8456342.pdf\" target=\"_blank\"  class=\"qodef-btn qodef-btn-medium qodef-btn-solid btnWebRosa2\"  >\n    <span class=\"qodef-btn-text\">T\u00e9l\u00e9charger l&#039;article<\/span>\n    <\/a>[\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p><strong>Res Int. 2019 Feb 10;2019:8456342.<\/strong><br \/>\nDental implantology allows replacement of failing teeth providing the patient with a general improvement of health. Unfortunately not all reconstructions succeed, as a consequence of the development of infections of bacterial origin on the implant surface. Surface topography is known to modulate a differential response to bacterial and mammalian cells but topographical measurements are often limited to vertical parameters.<\/p>\n","protected":false},"author":2002,"featured_media":43609,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[151],"tags":[],"class_list":["post-43619","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-implantologie-orale"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/posts\/43619","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/users\/2002"}],"replies":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/comments?post=43619"}],"version-history":[{"count":1,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/posts\/43619\/revisions"}],"predecessor-version":[{"id":43620,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/posts\/43619\/revisions\/43620"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/media\/43609"}],"wp:attachment":[{"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/media?parent=43619"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/categories?post=43619"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/tags?post=43619"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}