{"id":43057,"date":"2024-08-26T10:37:15","date_gmt":"2024-08-26T09:37:15","guid":{"rendered":"https:\/\/www.btitrainingcenter.com\/assessing-peri-implant-bacterial-community-structure-the-effect-of-microbiome-sample-collection-method\/"},"modified":"2025-08-18T11:07:11","modified_gmt":"2025-08-18T10:07:11","slug":"assessing-peri-implant-bacterial-community-structure-the-effect-of-microbiome-sample-collection-method","status":"publish","type":"post","link":"https:\/\/www.btitrainingcenter.com\/fr\/assessing-peri-implant-bacterial-community-structure-the-effect-of-microbiome-sample-collection-method\/","title":{"rendered":"Assessing peri-implant bacterial community structure: the effect of microbiome sample collection method"},"content":{"rendered":"

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Abstract<\/h3>\n
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Background:\u00a0<\/strong>Oral microbiota comprises polymicrobial communities shaped by mutualistic coevolution with the host, contributing to homeostasis and regulating immune function. Nevertheless, dysbiosis of oral bacterial communities is associated with a number of clinical symptoms that ranges from infections to oral cancer. Peri-implant diseases are biofilm-associated inflammatory conditions affecting the soft and hard tissues around dental implants. Characterization and identification of the biofilm community are essential for the understanding of the pathophysiology of such diseases. For that sampling methods should be representative of the biofilm communities Therefore, there is a need to know the effect of different sampling strategies on the biofilm characterization by next generation sequencing.<\/p>\n

Methods:\u00a0<\/strong>With the aim of selecting an appropriate microbiome sampling procedure for periimplant biofilms, next generation sequencing was used for characterizing the bacterial communities obtained by three different sampling strategies two months after transepithelial abutment placement: adjacent periodontal crevicular fluid (ToCF), crevicular fluid from transepithelial abutment (TACF) and transepithelial abutment (TA).<\/p>\n

Results:\u00a0<\/strong>Significant differences in multiple alpha diversity indices were detected at both the OTU and the genus level between different sampling procedures. Differentially abundant taxa were detected between sample collection strategies, including peri-implant health and disease related taxa. At the community level significant differences were also detected between TACF and TA and also between TA and ToCF. Moreover, differential network properties and association patterns were identified.<\/p>\n

Conclusions:\u00a0<\/strong>The selection of sample collection strategy can significantly affect the community composition and structure.<\/p>\n<\/div>\n

Keywords:\u00a0<\/strong>Crevicular fluid; Diversity; Metagenomics; Oral microbiota; Transepithelial abutment.<\/p>\n<\/div>\n

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Figures<\/h3>\n

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BMC Oral Health. 2024 Aug 26;24(1):1001.<\/strong>
\nOral microbiota comprises polymicrobial communities shaped by mutualistic coevolution with the host, contributing to homeostasis and regulating immune function. Nevertheless, dysbiosis of oral bacterial communities is associated with a number of clinical symptoms that ranges from infections to oral cancer. Peri-implant diseases are biofilm-associated inflammatory conditions affecting the soft and hard tissues around dental implants. Characterization and identification of the biofilm community are essential for the understanding of the pathophysiology of such diseases. For that sampling methods should be representative of the biofilm communities Therefore, there is a need to know the effect of different sampling strategies on the biofilm characterization by next generation sequencing.<\/p>\n","protected":false},"author":2002,"featured_media":43010,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[151],"tags":[],"class_list":["post-43057","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-implantologie-orale"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/posts\/43057","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/users\/2002"}],"replies":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/comments?post=43057"}],"version-history":[{"count":1,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/posts\/43057\/revisions"}],"predecessor-version":[{"id":43058,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/posts\/43057\/revisions\/43058"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/media\/43010"}],"wp:attachment":[{"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/media?parent=43057"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/categories?post=43057"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/fr\/wp-json\/wp\/v2\/tags?post=43057"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}