{"id":20584,"date":"2015-03-30T09:00:45","date_gmt":"2015-03-30T09:00:45","guid":{"rendered":"https:\/\/dev2.btitrainingcenter.com\/leukocyte-plasma-more-pro-inflammatory-environment\/"},"modified":"2025-04-09T12:22:18","modified_gmt":"2025-04-09T11:22:18","slug":"leukocyte-plasma-more-pro-inflammatory-environment","status":"publish","type":"post","link":"https:\/\/www.btitrainingcenter.com\/de\/leukocyte-plasma-more-pro-inflammatory-environment\/","title":{"rendered":"Leukocyte inclusion within a platelet rich plasma-derived fibrin scaffold stimulates a more pro-inflammatory environment and alters fibrin properties"},"content":{"rendered":"

[vc_row content_text_aligment=”left” row_background_lines=”no”][vc_column][vc_column_text]<\/p>\n

Abstract<\/h3>\n
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One of the main differences among platelet-rich plasma (PRP) products is the inclusion of leukocytes that may affect the biological efficacy of these autologous preparations. The purpose of this study was to evaluate whether the addition of leukocytes modified the morphological, biomechanical and biological properties of PRP under normal and inflammatory conditions. The release of pro-inflammatory cytokines from plasma rich in growth factors (PRGF) and leukocyte-platelet rich plasma (L-PRP) scaffolds was determined by enzyme-linked immunosorbent assay (ELISA) and was significantly increased under an inflammatory condition when leukocytes were included in the PRP. Fibroblasts and osteoblasts treated with L-PRP, under an inflammatory situation, underwent a greater activation of NF\u0138B pathway, proliferated significantly less and secreted a higher concentration of pro-inflammatory cytokines. These cellular events were assessed through Western blot and fluorimetric and ELISA methods, respectively. Therefore, the inclusion of leukocytes induced significantly higher pro-inflammatory conditions.<\/p>\n<\/div>\n

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Figures<\/h3>\n

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PLoS One. 2015 Mar 30;10(3):e0121713.<\/strong> One of the main differences among platelet-rich plasma (PRP) products is the inclusion of leukocytes that may affect the biological efficacy of these autologous preparations. The purpose of this study was to evaluate whether the addition of leukocytes modified the morphological, biomechanical and biological properties of PRP under normal and inflammatory conditions.<\/p>\n","protected":false},"author":1,"featured_media":15411,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[156],"tags":[647,410,411,655,412],"class_list":["post-20584","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-regenerative-medizin","tag-endoret-en-de","tag-implantologia-de","tag-medicina-de","tag-prgf-en-de","tag-salud-de"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/posts\/20584","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/comments?post=20584"}],"version-history":[{"count":1,"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/posts\/20584\/revisions"}],"predecessor-version":[{"id":37530,"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/posts\/20584\/revisions\/37530"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/media\/15411"}],"wp:attachment":[{"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/media?parent=20584"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/categories?post=20584"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.btitrainingcenter.com\/de\/wp-json\/wp\/v2\/tags?post=20584"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}